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In isopycnic separations, particle density alone establishes remaining position from the density gradient. Figure 12-nine compares the sedimentation coefficients and densities of the variety of cellular components.
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Depending upon the efficiency from the homogenization course of action, some unbroken cells and huge mobile fragments may additionally be recovered Within this portion.
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In the instance given previously mentioned, the Original nuclear fraction would consist of some mitochondria, microsomes, and cytosol; the mitochondrial fraction received in the 2nd centrifugation will be contaminated with microsomes and cytosol, and the like.
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The most widely used methods for identifying the constituents of fractions made by centrifugation contain microscopy and chemical and bioassays for unique markers.
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